Figure 5.

Transcriptome analysis: TGFβ effects in VPP-induced neurodegeneration. (A). Heatmap showing the deviation of expression from the mean of all significantly dysregulated genes in any of the pairwise comparisons: VPP and double mutant, respectively, against control retinae. Significantly dysregulated genes, which were significantly dysregulated (up- or down-regulated) in the same way in both comparisons are not shown here, but can be found in Figure S3. R21742-R21761 = RNAseq sample number. (B). Visualization of the Reactome MAPK targets/ Nuclear events mediated by MAP kinases signaling pathway. We converted the Reactome pathway into functional interaction networks. For each network, genes were colored according to the comparison (see legend) and their dysregulation state (white—not significantly dysregulated; red—significantly upregulated; and blue—significantly downregulated). (C). Violin blots with individual data points showing Hddc3 gene expression data from the RNAseq as normalized counts for control, Tgfbr2^ΔOC, VPP and double mutant animals. ANOVA with Bonferroni post-hoc analysis; *** p < 0.001.