Table 1.

Assembly statistics of Nothobranchius guentheri transcriptome according to QUAST, rnaQUAST, and BUSCO metrics.

QUAST Metrics	Joint Dataset, 115 Gb	Current Dataset, 29 Gb	Dataset of the Work [22], 38 Gb
Transcripts (>0 bp)	595,883	226,597	352,297
Transcripts (>500 bp)	184,805	89,658	127,262
Transcripts (>1000 bp)	81,223	50,507	66,471
Transcripts (>5000 bp)	5757	3769	6322
Transcripts (>10,000 bp)	500	299	604
Transcripts (>25,000 bp)	10	6	5
Total length (>0 bp)	386,870,887	195,140,104	285,906,387
Total length (>500 bp)	263,650,013	153,792,134	217,410,128
Total length (>1000 bp)	192,720,831	126,643,405	175,623,569
Total length (>5000 bp)	40,282,776	26,395,594	45,099,163
Total length (>10,000 bp)	6,597,510	4,117,143	7,821,995
Total length (>25,000 bp)	268,609	162,941	134,557
Largest transcript	27,943	27,845	27,376
N50	1927	2423	2539
N75	948	1283	1231
L50	36,670	18,767	24,581
L75	86,380	40,530	55,134
% Length (>500 bp)	68.15	78.81	76.04
% Length (>1000 bp)	49.82	64.90	61.43
% Length (>5000 bp)	10.41	13.53	15.77
% Length (>10,000 bp)	1.71	2.11	2.74
% Length (>25,000 bp)	0.07	0.08	0.05
rnaQUAST metrics
Aligned, %	99.29	99.63	99.78
Uniquely aligned, %	93.74	94.16	95.04
Multiply aligned, %	2.05	2.23	1.03
Unaligned, %	0.71	0.37	0.22
Avg. aligned fraction	0.9860	0.9880	0.9850
Avg. alignment length	615.47	818.24	769.95
Avg. mismatches per transcript	2.90	1.99	3.00
Misassemblies	20 848	7 336	13 051
Misassemblies per 1 Mb	53.89	37.59	45.65
Completed BUSCOs, %
Cyprinodontiformes	66.96	59.19	63.94
Actinopterygii	88.38	82.83	86.68
Vertebrata	90.58	87.75	90.88
Metazoa	97.17	97.27	97.69
Eukaryota	96.86	96.08	98.04
BUSCO duplication ratio, %
Cyprinodontiformes	33.49	29.16	36.52
Actinopterygii	32.58	30.38	37.75
Vertebrata	33.25	32.18	39.37
Metazoa	28.26	26.40	32.62
Eukaryota	25.10	32.65	33.60
cell colors	worse	middle	better