Figure 5.

Inducing effects of α-T3E on ER stress in H2452 cells. (a) Cells were treated with α-T3E (20 µM) for 24 h, and the mRNA levels of ER-stress-related molecules were assessed by real-time quantitative PCR. The data are means ± SD, n = 3. * p < 0.05, ** p < 0.01 vs. the control. (b) Cells were treated with α-T3E (20 µM) for the indicated periods, and the levels of ER-stress-related proteins were measured by immunoblotting. The α-Tubulin protein levels served as the loading control. (c) A densitometric analysis of ER-stress-related proteins was performed. The data are means ± SD, n = 3. * p < 0.05 vs. the control.