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Secretion of MoPtep1 signal peptide. (A) The secretion of MoPtep1 signal peptide was detected by yeast signal trap assay. (B) TTC assay of MoPtep1 signal peptide. The recombinants pSUC2-MoPtep1-FL (full-length sequence) and pSUC2-MoPtep1-NS (no signal peptide sequence) were transformed into yeast strain YTK12. pSUC2-Avr1b was positive control, pSUC2-mg87 and pSUC2-empty vector (EV) as a negative control. All experiments were repeated three times.
