Figure 1.

Trichostatin A (TSA) changed the calcium pyrophosphate (CPP)-related gene expressions in transforming growth factor (TGF)-β1-treated chondrocytes. Human primary chondrocytes (HC-a cells) were pretreated with different concentrations of TSA (100–800 nM) for 30 min and then treated with 5 ng/mL TGF-β1 for 24 h. (A) Total RNAs were extracted, and mRNA expression levels of ANKH, ENPP1, and TNAP were determined by real-time PCR. Data are expressed as the mean ± SE of five independent experiments. * p < 0.05 vs. column 1; # p < 0.05 vs. column 2. (B) Total cellular proteins were collected, and protein expressions were detected by Western blotting.