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Quantitative real-time polymerase chain reaction (PCR) analysis of BRITER cells on diluted each bone block substitute extract solution media. Osteoblast differentiation was confirmed by qPCR analysis by osteoblast differentiation marker genes (a) ALP, (b) Osterix, and (c) Osteocalcin. ALP, Osterix, and Osteocalcin normalized to β-actin (n.s represents no significance, *** p < 0.001).
