Figure 4.

Cell cycle analysis of cytotoxicity induction by 4,6,8-trimethyl azulene amide derivatives. Ca9-22 cells were incubated for 24 h with the indicated concentrations of test samples and subjected to a cell sorter. (A) Representative cell cycle distribution pattern; (B) distribution into subG₁, G₁, S and G₂/M phase. Each value represents the mean ±S.D. of triplicate determinations. * p < 0.05 between control and sample, examined by one-way ANOVA and Dunnett’s post-test.