Table 2.

Neurotoxicity of 4,6,8-trimethyl azulene amide derivatives and reference compounds.

	CC50 (μM)
	Exp. 1				Exp. 2				Exp. 3
	PC12	SH-SY5Y	LYPPB6	Mean	PC12	SH-SY5Y	LYPPB6	Mean	dPC12	Normal Cells	NT 1
				(E)				(F)	(G)	(D)	(D/E)	(D/F)	(D/G)
7	59	400	400	286	68	400	400	289	400	386	1.4	1.3	1.0
9	35	73	56	55	36	63	74	58	19	189	3.5	3.3	10.2
15	378	42	400	273	400	400	400	400	400	393	1.4	1.0	1.0
DOX	0.07	0.02	0.32	0.14	0.26	0.12	0.78	0.39	0.22	10	70.6	25.0	44.9
5-FU	14	5.85	250	89.95	2	1	125	43	297	1000	11.0	23.3	3.4
L-PAM	N.D.	N.D.	N.D.	N.D.	2	2	25	10	26	191	N.D.	19.8	7.2

¹ Neurotoxicity (NT) was determined by dividing the mean values of CC₅₀ against normal human oral cells (D) by the mean of CC₅₀ against PC12, SH-SY5Y and LYPPB6 (E in Exp. 1 and F in Exp. 2) or the CC₅₀ against differentiated PC12 cells (G). Dose–response curve from which the CC₅₀ values were delineated in Figure S5. N.D., not determined.