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Effect of Ca2+ and EGTA in the relative mobility of calmodulin. (A) SDS-PAGE of calmodulin (CaM) pre-incubated with either Ca2+ or EGTA. (B) Native-PAGE of CaM pre-incubated with either Ca2+ or EGTA. In both cases, carbonic anhydrase was included as external loading control. Spot graphs show the relative mobility (mean, SEM) of the denatured (SDS-PAGE) or the native (Native-PAGE) CaM. Data of 3 independent experiments were analyzed with a two-sample t test, * p < 0.05.
