Figure 4.

Calmodulin kinase II activity determined in the presence of melatonin. (A) Basal calmodulin kinase II (CaMKII) activity in the presence of calmodulin (CaM) with EGTA or Ca²⁺ in aqueous or lipidic microenvironment. (B) Percentage of the difference ($\Delta y_i\%$) between CaMKII basal activity and the activity of CaMKII assessed in the presence of Ca²⁺-CaM and four melatonin (MEL) concentrations (10⁻¹¹, 10⁻⁹, 10⁻⁷, 10⁻⁵ M) in aqueous or lipidic media. Zero is the activity of CaMKII determined with Ca²⁺-CaM and the vehicle (Illustrated as a punctuated line). A one-way or two-way ANOVA and a Tukey multiple comparisons test were performed. Significant differences (p < 0.05) between the vehicle (aqueous or lipidic) and different MEL concentrations are indicated with (*), and (#) indicates global differences between aqueous and lipidic environments. Different literals p < 0.05. Results are represented as box and whiskers with the median, first and third quartile with maximum and minimum values of at least 4 experimental repetitions.