Figure 5.

TAM inhibited PAs progression both in vitro and in vivo. (A) IC₅₀ values were determined by CCK8 assay after GH3 and AtT-20 cells treatment with TAM. (B) The treatment of TAM reduced the levels of GH and ACTH secreted by the GH3 and AtT-20 cells respectively. (C) Xenograft mouse model establishment and TAM administration. The panel showed representative tumor tissues of animals at the end of treatment. (D) TAM inhibited tumor growth as measured by tumor volume. (E) TAM inhibited tumor growth as measured by tumor weight. (F) The secretion levels of GH in the plasma were detected using an enzyme-linked immunosorbent assay. NC normal control, sham mice treated with the vehicle. VC vehicle control, refers to tumor-bearing mice treated with the vehicle. Bars indicates SEM, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control, VC or NC. TAM: tamoxifen; PAs: pituitary adenomas; GEFI: gefitinib; TMZ: temozolomide.