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TRPC7 biosensors were designed to detect their conformational changes in a BRET2 assay. The double brilliance intramolecular BRET TRPC7 biosensor was double-tagged N-terminally with GFP10 and C-terminally with RLucII (or N-term RLucII and C-term GFP10). TRPC7, Transient receptor potential canonical channel 7; RlucII, Renilla luciferase; GFP10, green fluorescent protein.
