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. 2022 Feb 24;23(5):2502. doi: 10.3390/ijms23052502

Figure 3.

Figure 3

Double brilliance GFP10-TRPC7-RLucII biosensor also shows intermolecular interactions between TRPC7 subunits. HEK293 cells were co-transfected with plasmids encoding GFP10-TRPC7-RLucII (75 ng), Gαq-DREADD (500 ng) and increasing concentration of the corresponding untagged TRPC7 WT (a) or TRPC3 WT (b). Basal BRET ratio was measured. Statistical analyses were performed using a Kruskal–Wallis multiple comparisons followed by a Dunn’s post-hoc test. ** p < 0.01, *** p < 0.001 compared to no competition (0 ng of TRPC7 or TRPC3 WT). (c,d) HEK293 cells were co-transfected with plasmids encoding Gαq-DREADD (500 ng), GFP10-TRPC7-RLucII (75 ng), and TRPC7 WT (500 ng) (c) or TRPC3 WT (500 ng) (d) and were stimulated with CNO (1 µM) or vehicle as a control. BRET signal was measured for 10 min. Each data set represents the mean of three independent experiments, which were each performed in triplicate, and expressed as the mean ± S.E.M. Statistical analyses were performed using a two-way ANOVA with multiple comparisons followed by a Sidak’s post-hoc test. * p < 0.05, *** p < 0.001 for control vs. CNO.