Figure 4.

Gα_q-coupled receptors modulate BRET ratio of GFP10-TRPC7-RLucII biosensor. (a–c) HEK293 cells co-transfected with plasmids encoding GFP10-TRPC7-RlucII biosensor (75 ng) and Gα_q-coupled AT₁R (a), TPα (b), or UT (c) receptors (500 ng) and were respectively stimulated with AngII, U-46619 and Urotensin (1 µM) or vehicle as a control. BRET signal was measured for 10 min. Each data set represents the mean of three independent experiments, which were each performed in triplicate, and expressed as the mean ± S.E.M. Statistical analyses were performed using a two-way ANOVA with multiple comparisons followed by a Sidak’s post-hoc test. * p < 0.05 for control vs. stimulation.