Figure 6.

Stimulation of endogenous AT₁R induces GFP10-TRPC7-RLucII biosensor conformational changes. Isolated rat neonatal cardiac fibroblasts were infected with the adenovirus encoding GFP10-TRPC7-RlucII biosensor. (a) Cells were stimulated with AngII (1 µM) 48 h post-infection, and BRET signal was measured for 10 min. Cells were pre-incubated with Losartan (100 µM) (b), YM-254890 (1 µM) (c) or Y27632 (10 µM) (d) before BRET measurement, and stimulation with AngII (1 µM) or vehicle as a control. BRET signal was measured for approximatively 10 min. Each data set represents the mean of three independent experiments, which were each performed in triplicate, and expressed as the mean ± S.E.M. Statistical analyses were performed using a two-way ANOVA with multiple comparisons, followed by a Sidak’s post-hoc test. * p < 0.05, ** p < 0.01 for control vs. AngII and $ p < 0.05 for Y27632 vs. AngII + Y27632.