TABLE 1.

Summary of the four physiological states of C. albicans as a consequence of incubation with increasing concentrations of amphotericin B (AmB) for 10 h

Method of evaluation	Physiological parameter measured	Result at AmB concn (μg/ml) ofa:
		0–0.5	0.5–1.0	1.0–2.0	2.0–4.0
Agar plate counts (<99% inhibition of control)	Replication competency	Yes	No	No	No
Intracellular ATP (<99% inhibition of control)	Metabolic potential	Yes	No	No	No
Vitality dyes
DiOC6(3)	Negative membrane potential	Yes	No	No	No
CFDA	Intracellular enzyme activity	Yes	No	No	No
Mortality dyes
DiBAC4(3)	Positive membrane potential	No	No	Yes	Yes
SYBR Green I	Loss of membrane integrity	No	No	No	Yes

^aYes, positive result for the physiological parameter being measured; no, negative result for the physiological parameter being measured.