Figure 3.

Expression of HIF-1/2α regulatory proteins in the placentae of complicated pregnancies. Expression of the HIF-α regulatory proteins PHD1 (A), PHD2 (B), and PHD3 (C) was assessed by immunoblotting in placentae of normal (n = 13), IUGR (n = 16) and PE (n = 19) pregnancies. Representative Western blot and densitometric analysis of expression are shown. β-actin is included as a loading control. (D) Function of PHD enzymes was assessed by immunoblotting for hydroxylated P⁴⁰² and P⁵⁶⁴ in HIF-1α. In this case immunoblotting for HIF-1α was used as the loading control. The value of one normal pregnancy control was normalised to one and changes observed in the other samples shown as a fold change compared to this reference sample. Data represent mean ± SEM. p values as compared to normal pregnancy are indicated. NS = Not significant. Full gels for immunoblots can be found in SF3 and SF4.