Table 2.

PCR primers and conditions used for oxygen sensing/response genes.

Gene	Primers		[Mg2 + ] mM	Annealing Temp	Product Length	Cycle Number
sFlt-1	SAS	5′ gcaccttggttgtggctgact 3′ 5′ gagcccgggggtctcattatt 3′	1.25	60 °C	643 bp	25
HIF-1α	SAS	5′ cgttgtgagtggtattattcagcacg 3′ 5′ ggtcatcagtttctgtgtcgttgc 3′	1.5	63 °C	248 bp	31
HIF-2α	SAS	5′ gctcctctcctcagtttgctc 3′ 5′ ctgttagctccacctgtgtaagtc 3′	1.5	63 °C	179 bp	30
β-actin	SAS	5′ gaggcccagagcaagagag 3′ 5′ ccagaggcgtacagggatag 3′	1.5	57 °C	200 bp	20