Effect of THC and CBD treatment alone or in combination on Aβ1–40 and Aβ1–42 production in N2a/APPswe cells at 24 (A) for Aβ1–40; (B) for Aβ1–42) and 42 (C) for Aβ1–40; (D) for Aβ1–42) hours after the treatment. The production of Aβ1–40 and Aβ 1–42 in the cell culture supernatant was determined by ELISA. After N2a/APPswe cells were treated with THC and CBD alone or in combination for 24 h, Aβ1–40 and Aβ 1–42 levels in the supernatant reduced significantly by 16~32% and 18~35%, respectively, compared with those in the non-treated control samples. No significant changes in the Aβ1–40 and Aβ 1–42 levels in the supernatant were found between the non-treated and treated samples after the 42-h treatment. Data are expressed as mean ± SD (N = 4). SD is denoted by the error bars. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the control group and + p < 0.05 compared with the 100 nM THC treatment group using one-way ANOVA followed by the Tukey–Kramer post hoc multiple comparison test.