Figure 3.

Evaluation of the baseline spatial reference memory of 14-month-old APP/PS1 mice in three study groups (i.e., the control transgenic control (TG), 0.02 mg/kg and 0.2 mg/kg THC treatment groups as well as 14-month-old non-transgenic (NTG) mice using the radial arm water maze (RAWM) test. Individual mice were subjected to five blocks of trials each day for three days with each block containing three trials. (A) No significant difference in baseline latency between male and female mice within individual study groups (p = 0.56, p = 0.06, p = 0.51 and p = 0.947 for NTG, TG, 0.02 mg/kg and 0.2 mg/kg THC groups, respectively, using the Independent sample t test). (B) No significant difference in baseline number of errors between male and female mice within individual study groups (p = 0.356, p = 0.299, p = 0.513, and p = 0.513 for NTG, TG, 0.02 mg/kg, and 0.2 mg/kg THC groups, respectively, using the Independent sample t test). (C) Significant increase in the baseline latency in TG (p < 0.01), 0.02 mg/kg THC (p < 0.05), and 0.2 mg/kg THC (p < 0.001) groups compared with the NTG control group in Trial 3 of the last block on day 3. (D) Significant increase in the baseline latency in TG (p < 0.01), 0.02 mg/kg THC (p < 0.05), and 0.2 mg/kg THC (p < 0.001) groups compared with the NTG control group in Trial 3 of the last block on day 3. Data are expressed as mean ± SD. SD is denoted by the error bars. A comparison of mean latency and number of errors between female and male animals in individual study groups was made with multiple t-tests with correction for multiple comparisons using the Holm–Sidak method. Comparison of mean baseline latency and number of errors among different study groups were conducted using one-way ANOVA with the post hoc Bonferroni’s multiple comparisons test. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared between the control NTG (N = 7), control TG (N = 6), 0.02 mg/kg THC (N = 8), and 0.2 mg/kg THC (N = 6) groups.