Table 6.

Adjusted mean cell type composition based on methylation at 12 years of age by gestational PFAS concentration (ng/mL) tercile in the HOME Study (Cincinnati, Ohio; enrolled 2003–2006).

PFAS [tercile (range)]	Cell type
	B cell (%)	CD4 (%)	CD8 (%)	Monocyte (%)	Neutrophil (%)	NK (%)	CD4/CD8	NLR	LMR
PFOA
T1 (1.1–4.2)	8.0	17.0	13.0	7.5	49.0	5.4	1.37	1.19	6.20
T2 (4.2–6.3)	9.0	18.0	13.0	7.7	48.0	5.0	1.52	1.13	6.41
T3 (6.3–17.4)	8.0	17.0	13.0	7.1	51.0	4.7	1.39	1.29	6.26
$p_{\text{Trend}}$a	0.2	0.61	0.64	0.56	0.14	0.089	0.17	0.17	0.84
PFOS
T1 (1.4–10.0)	9.0	18.0	13.0	7.0	47.0	5.3	1.38	1.12	6.63
T2 (10.0–16.0)	8.0	17.0	13.0	8.0	50.0	4.6	1.40	1.28	5.74
T3 (16.0–57.2)	8.0	18.0	13.0	7.0	50.0	5.2	1.50	1.22	6.49
$p_{\text{Trend}}$a	0.14	0.85	0.38	0.56	0.23	0.61	0.22	0.22	0.70
PFHxS
T1 (0.1–1.0)	9.0	18.0	13.0	8.0	48.0	5.0	1.40	1.14	6.23
T2 (1.0–2.0)	8.0	17.0	13.0	7.0	50.0	5.0	1.48	1.26	6.28
T3 (2.0–32.5)	8.0	17.0	13.0	7.0	49.0	5.0	1.40	1.22	6.37
$p_{\text{Trend}}$a	0.23	0.28	0.39	0.41	0.23	0.29	0.19	0.19	0.58
PFNA
T1 (0.3–0.7)	8.0	17.0	13.0	7.0	49.0	5.0	1.41	1.20	6.28
T2 (0.7–1.1)	8.0	17.0	13.0	8.0	50.0	5.0	1.37	1.25	6.10
T3 (1.1–2.9)	8.0	19.0	13.0	7.0	48.0	5.0	1.54	1.14	6.59
$p_{\text{Trend}}$a	0.62	0.26	0.84	0.99	0.72	0.9	0.74	0.74	0.42

Note: All models were adjusted for child sex, child age, maternal pregnancy cotinine concentrations, race, and annual household income. CD4, T helper cells; CD8, cytotoxic T cells; CD4/CD8, the ratio of T helper cells to cytotoxic T cells; HOME, Health Outcomes and Measures of the Environment; NK, natural killer cells; NLR, ratio of neutrophils to lymphocytes; LMR, the ratio of lymphocytes to monocytes; PFAS, perfluoroalkyl substances; PFHxS, perfluorohexane sulfonate; PFNA, perfluorononanoate; PFOA, perfluorooctanoate; PFOS, perfluorooctane sulfonate.

^a The $p_{\text{Trend}}$ for cell type composition differences across median PFAS concentrations in each tercile.