Figure 1.

PNA-PCR clamping experimental design. Amplification of SF3B1 is performed in presence of the PNA probe (yellow), designed on the WT sequence, and the primer competitor (black), designed on the mutated sequence. In these conditions, the PCR of the WT sequence is inhibited by the hybridization of PNA/DNA. In contrast, in the presence of the SF3B1 p.Lys700Glu genotype, the primer/DNA duplex allows the amplification of the target sequence.