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Time kinetics study from 4–72 h of PRGF-induced genes in primary normal human epidermal keratinocytes (NHEKs). Human primary keratinocytes were stimulated with PRGF (1:10) for the indicated periods. Relative gene expression was analyzed by real-time PCR. Shown are means ± s.e.m of three independent stimulations (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns = non-significant; Student’s t-test).
