Table 1.

Sequences of specific primers for RT-PCR analyses.

Genes	Sequences 5′-3′
ACAN	Fwd: CAA-CCT-CCT-GGG-TGT-AAG-GA
	Rev: TGT-AGC-AGA-TGG-CGT-CGT-AG
Ank	Fwd: CAA-GAG-AGA-CAG-GGC-CAA-AG
	Rev: AAG-GCA-GCG-AGA-TAC-AGG-AA
Sox-9	Fwd: CTG-AAG-AAG-GAG-AGC-GAG-GA
	Rev: GGT-CCA-GTC-ATA-GCC-CTT-CA
Col 2	Fwd: TCC-CTC-TGG-TTC-TGA-TGG-TC
	Rev: CTC-TGT-CTC-CAG-ATG-CAC-CA
Enpp1	Fwd: TAT-GCC-CAA-GAA-AGG-AAT-GG
	Rev: GCA-GCT-GGT-AAG-CAC-AAT-GA
RP29	Fwd: CTC-TAA-CCG-CCA-CGG-TCT-GA
	Rev: ACT-AGC-ATG-ATT-GGT-ATC-AC

ACAN—aggrecan; Ank—inorganic pyrophosphate transport regulator; Sox-9—SRY (Sex-Determining Region Y)-Box 9; Col 2—type II collagen; Enpp1—ectonucleotide pyrophosphatase/phosphodiesterase 1; Fwd—forward primer; RT-PCR—real-time polymerase chain reaction; Rev—reverse primer; RP—ribosomal protein.