Table 12.

qPCR primers selected using publicly available Web service PrimerBLAST [240].

No.	Gene	Forward, 5′→3′	Reverse, 5′→3′
DEGs identified in hippocampus of tame versus aggressive adult male rats [this work]
1	Ascl3	CCTCTGCTGCCCTTTTCCAG	ACTTGACTCGCTGCCTCTCT
2	Defb17	TGGTAGCTTGGACTTGAGGAAAGAA	TGCAGCAGTGTGTTCCAGGTC
Reference genes
3	B2m	GTGTCTCAGTTCCACCCACC	TTACATGTCTCGGTCCCAGG
4	Hprt1	TCCCAGCGTCGTGATTAGTGA	CCTTCATGACATCTCGAGCAAG
5	Ppia	TTCCAGGATTCATGTGCCAG	CTTGCCATCCAGCCACTC
6	Rpl30	CATCTTGGCGTCTGATCTTG	TCAGAGTCTGTTTGTACCCC

Notes. Regarding the DEGs subjected to this qPCR verification, see Table 2; reference rat genes: B2m, β-2-microglobulin [241]; Hprt1, hypoxanthine phosphoribosyltransferase 1 [242]; Ppia, peptidylprolyl isomerase A [243]; Rpl30, ribosomal protein L30 [244].