Table 2.
Antioxidant activities of seven C. aurantium cultivars EO and their combinations.
| DPPH (IC50 μg/mL) |
ABTS (IC50 μg/mL) |
β-Carotene Bleaching Test (IC50 μg/mL) |
FRAP μM Fe2+/g |
|
|---|---|---|---|---|
| C. aurantium EO | ||||
| C1 | 38.39 ± 2.12 g | 25.31 ± 2.66 a | 38.61 ± 3.54 n | 20.62 ± 2.36 n |
| C2 | 33.01 ± 1.71 c | 30.14 ± 2.27 d | 22.68 ± 2.16 g | 28.23 ± 4.12 h |
| C3 | 40.18 ± 2.82 i | 36.22 ± 2.42 j | 55.72 ± 3.87 v | 55.92 ± 2.92 a |
| C4 | 33.98 ± 2.00 c | 27.45 ± 1.85 b | 15.46 ± 1.31 b | 44.44 ± 3.66 d |
| C5 | 39.01 ± 2.74 h | 29.56 ± 2.93 c | 48.73 ± 2.64 q | 50.23 ± 3.41 b |
| C6 | 37.93 ± 2.54 f | 27.38 ± 1.77 b | 18.56 ± 1.56 d | 20.65 ± 1.78 n |
| C7 | 40.74 ± 3.18 i | 32.32 ± 2.72 f | 49.68 ± 2.93 r | 45.44 ± 2.45 c |
| EOs Combination (1:1 v/v) | ||||
| C1C2 | 36.12 ± 1.81 e | 38.39 ± 2.75 l | 57.28 ± 3.41 z | 19.98 ± 1.98 o |
| C1C3 | 42.09 ± 2.48 k | 33.01 ± 2.84 g | 25.77 ± 2.58 j | 21.72 ± 1.92 m |
| C1C4 | 31.75 ± 2.33 a | 40.18 ± 3.01 n | 50.63 ± 3.67 s | 23.08 ± 2.01 k |
| C1C5 | 32.64 ± 2.54 b | 33.98 ± 2.52 g | 31.96 ± 2.73 k | 27.18 ± 2.23 i |
| C1C6 | 31.05 ± 2.25 a | 39.01 ± 2.96 m | 53.82 ± 3.86 | 20.73 ± 2.14 m |
| C1C7 | 31.44 ± 2.63 a | 37.93 ± 2.84 kl | 13.42 ± 2.24 a | 22.60 ± 2.35 l |
| C2C3 | 37.16 ± 2.85 f | 39.42 ± 2.73 | 41.14 ± 3.72 p | 22.04 ± 2.32 l |
| C2C4 | 34.60 ± 2.16 d | 35.16 ± 2.4 i | 24.12 ± 1.94 i | 23.49 ± 2.41 k |
| C2C5 | 38.49 ± 2.92 g | 36.85 ± 2.64 j | 37.66 ± 2.57 m | 24.26 ± 2.66 j |
| C2C6 | 38.68 ± 2.73 g | 33.98 ± 2.25 g | 17.53 ± 2.01 c | 22.55 ± 2.47 l |
| C2C7 | 37.60 ± 2.77 f | 38.13 ± 2.74 l | 25.95 ± 2.43 j | 23.14 ± 2.52 k |
| C3C4 | 41.96 ± 3.24 j | 46.23 ± 3.88 o | 22.68 ± 2.52 g | 22.91 ± 2.36 l |
| C3C5 | 33.91 ± 2.45 c | 37.16 ± 3.57 k | 55.06 ± 3.04 v | 22.71 ± 2.2 l |
| C3C6 | 31.86 ± 2.24 a | 33.08 ± 2.35 g | 36.08 ± 2.86 l | 23.11 ± 2.31 k |
| C3C7 | 36.37 ± 2.57 e | 31.40 ± 2.49 e | 37.41 ± 2.97 m | 23.19 ± 2.47 k |
| C4C5 | 34.38 ± 2.35 d | 36.22 ± 2.71 j | 23.54 ± 2.03 h | 29.61 ± 3.05 g |
| C4C6 | 37.16 ± 2.76 f | 34.68 ± 2.52 h | 57.72 ± 3.81 z | 30.95 ± 3.16 f |
| C4C7 | 34.60 ± 2.40 d | 38.51 ± 2.73 l | 19.54 ± 1.61 e | 27.23 ± 2.42 i |
| C5C6 | 38.49 ± 3.48 g | 38.34 ± 2.65 l | 54.86 ± 3.33 u | 28.06 ± 2.77 h |
| C5C7 | 38.68 ± 3.39 g | 31.12 ± 2.44 e | 21.64 ± 1.57 f | 30.97 ± 3.26 f |
| C6C7 | 37.60 ± 2.98 f | 34.30 ± 2.25 h | 51.23 ± 3.58 t | 30.14 ± 3.01 f |
| C1 + C2 + C3 + C4 + C5 + C6 + C7 | 44.96 ± 3.70 l | 39.23 ± 2.73 m | 40.21 ± 3.29 o | 38.06 ± 3.54 e |
| Sign | ** | ** | ** | ** |
Data are expressed as means ± standard deviation (SD). The following positive controls were used: ascorbic acid in 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (IC50 value of 5.03 ± 0.79 μg/mL) and 2,2′-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic acid (ABTS) (IC50 value of 1.72 ± 0.09 μg/mL) test; propyl gallate in β-carotene bleaching test (IC50 value of 0.09 ± 0.004 μg/mL); butylated hydroxytoluene (BHT) in ferric reducing ability power (FRAP) (FRAP value 63.27 ± 4.48 μM Fe(II)/g). Sign: significance at ** p < 0.05. Results followed by different letters in the same line are significantly different according to Tukey’s multiple range test.