Figure 6.

Expressions of skin barrier-related proteins in the skins of mice treated with DNCB and 1-iodohexadecane. (a) Representative images. The dorsal skins of mice were treated with 2,4-dinitrochlorobenzene (DNCB) with or without 1-iodohexadecane (1-Iodo; 50 or 100 μg/mL) for 21 days. Skin tissues were excised, fixed with 4% formaldehyde, embedded in paraffin, and sectioned, and sections were then immunostained with anti-loricrin or -filaggrin as described in the Materials and Methods section. Scale bar = 100 μm. (b,c) Results obtained from panel (a). The expression levels of loricrin (upper images of panel (a), n = 3) and filaggrin (lower images of panel (a), n = 3) were measure using Image Pro Plus. Protein levels are expressed as percentages of those of DNCB-untreated controls. Dexamethasone (Dexa, 0.1%) was used as a positive control. Values are means ± SEMs. * p < 0.05 vs. the DNCB control group. Vehi, vehicle (100% olive oil).