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. 2022 Feb 28;27(5):1615. doi: 10.3390/molecules27051615

Figure 2.

Figure 2

(A) Tandem MS spectrum of a pepsin-generated peptide of TmFuc, revealing its labeling at W105. The annotated y and b ion series are shown above and below the peptide sequence, respectively. The y5 and b6 ions carry the mass increment of the probe (+350.16 Da), indicating the modification to be on the W105 residue. (B) Structure of TmFuc with its active-site catalytic acid D224 annotated in red, and the two residues modified by the quinone methide probe AH062 annotated in green (PDB code 1ODU).