Table 3.
List of primers used in the study.
| Primer/Probe | Sequence (5′ to 3′ Direction) | Final Concentration (μM) | Usage |
|---|---|---|---|
| StrVA | |||
| Forward | ACCTGGCCTTGTCYCGGC | 0.5 | sequencing of virus isolates |
| Reverse | WGGTGGWGGGGACGTACAAC | 0.5 | |
| 1750 Forward | GATGTCTGGTGATATGGACA | 0.5 | RT-PCR assay |
| 1751 Reverse | ACCAATTCTCTACATCGTGT | 0.5 | |
| Forward | ATCTGGGTGAAGRCGGGGT | 0.5 | qPCR |
| Reverse | CAAAACCCTCTCATAAGGTTRTCCA | 0.5 | |
| Probe | ACTGTGGATATCCCCGACTGTATTGT | 0.2 | |
| StrV-1 | |||
| Forward | AACGGATATTGTGGCGCRAA | 0.5 | qPCR |
| Reverse | CCTGATGTTGTTKATATARCTGAGRTC | 0.5 | |
| Probe 01 | AAACCTCTTACCATCATCTCGTAAA | 0.175 | |
| Probe 02 | AARCCCCTCACCATCATYTCGTAA | 0.175 | |
| SMYEV | |||
| Forward | CCCTCCTGACGTACACAACAACTG | 0.5 | qPCR |
| Reverse 01 | CCGTGAGGGAGGAGAATACGC | 0.25 | |
| Reverse 02 | CCGTGAGGGAGGAGAATACAC | 0.25 | |
| Probe | TACTCTAGTYGCCATCGAGGTACAGTGC | 0.2 | |
| SCV | |||
| Forward 01 | ACAGTRTGCGCTTTAGAGGTTGTT | 0.5 | qPCR |
| Forward 02 | ACAGTGTGCGCTTTAGAGGTTATTC | 0.5 | |
| Forward 03 | ACAGTGTGCGCTTTAGAAGTTGTTC | 0.25 | |
| Reverse 01 | ACCTGATTATCTCCCATYCCCATT | 0.5 | |
| Reverse 02 | ACTTGATTATCCCCCATCCCCA | 0.5 | |
| Probe 01 | TCTCAATAYGATTGTACATACCGCAT | 0.15 | |
| Probe 02 | TCTCAATACGATTGCACATATCGCAT | 0.15 | |
| SVBV | |||
| Forward | AATATCTGTCTTTACTTGATSATGAACTTG | 0.5 | qPCR |
| Reverse 01 | CGTCTTCGCTGCTGCTGTAGTC | 0.25 | |
| Reverse 02 | CATCTTCACTGCTGCTGCTGTAG | 0.25 | |
| Probe | AGTTACAGGTACTTGTAGCAAAAGARATGA | 0.2 | |
| SMoV | |||
| Forward | GTAGGACACCGGCTCTTGGYAGT | 0.5 | qPCR |
| Reverse | TTGGRTCGTCACCTGAYCTCG | 0.5 | |
| Probe | ACAGGWGGCACTGTTTACAGTGTTCC | 0.25 | |
| SPV1 | |||
| Forward | CAACTGGGGTCGTACACTCGC | 0.5 | qPCR |
| Reverse | GGCCAGCCGAATCCTTTGAC | 0.5 | |
| Probe | ACCTACCCTGAACTCGCCGAACA | 0.2 | |
| Nad5 | |||
| Forward | GATGCTTCTTGGGGCTTCTTGTT | 0.25 | qPCR |
| Reverse | ACATAAATCGAGGGCTATGCGG | 0.25 | |
| Probe | CCACAATTAACATCACTACGGTCGGGCTA | 0.2 | |