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. 2022 Mar 4;14(5):1078. doi: 10.3390/nu14051078

Figure 1.

Figure 1

Lemon myrtle extract (LM) promotes BrdU incorporation into skeletal muscle satellite cells (SCs) but not in myoblasts in vitro. (a) Effects of LM and recombinant human hepatocyte growth factor (HGF) treatment on BrdU incorporation into SCs. (b) Effects of LM treatment on BrdU incorporation into L6 rat myoblast cells (left), and C2C12 mouse myoblast cells (right). SCs and myoblast cells were treated with LM or HGF for 22 h and then incubated with BrdU for an additional 2 h. The concentration of LM used is indicated in the figure. The HGF concentration used was 5 ng/mL. BrdU-positive (BrdU+) cells were detected by immunocytochemistry and the number of cells was counted. BrdU incorporation into each cell is expressed as BrdU+ cells per total cells and is shown as a fold change compared to the control (Ctrl). Data represent the mean ± standard deviation (SD) of three independent cultures. Significant differences (p < 0.05) relative to the Ctrl are indicated in the figure, and non-significant differences (p > 0.05) are not shown in the figure.