Table 2.
Primers for quantification of antioxidant mRNAs by qPCR.
| mRNA | 5′—————3′ | Forward/Reverse | Amplicon Size (Base Pairs) |
|---|---|---|---|
| GCLC | AACACAGACCCAACCCAGAG TGGCACATTGATGACAACCT |
Forward Reverse |
143 |
| GCLM | TGTGTGATGCCACCAGATTT GCTTCAATGTCAGGGATGCT |
Forward Reverse |
137 |
| HMOX1 | TTACCTTCCCGAACATCGAC TCCTCTGTCAGCATCACCTG |
Forward Reverse |
172 |
| Keap1 | GGCAGGACCAGTTGAACAGT ATCACTGTCCGGGTCATAGC |
Forward Reverse |
188 |
| Nrf2 | AGCAAGTTTGGCAGGAGCTA TTCTTTTTCCAGCGAGGAGA |
Forward Reverse |
164 |
| PRDX6 | AAACACCCACGGAAAAGTTG GTTTCTTGTCAGGGCCAAAA |
Forward Reverse |
153 |
| POR | GTCTTCTGCATGGCCACATA TGGCGTTGAAGTGCTCATAG |
Forward Reverse |
151 |
| B2M 1 | TGGTCTTTCTGGTGCTTGTC GGGTGGAACTGTGTTACGTAG |
Forward Reverse |
112 |
1 Reference mRNA. GCLC: Glutamate-Cysteine Ligase Catalytic Subunit; GCLM: Glutamate-Cysteine Ligase Modifier Subunit; HMOX1: Heme Oxygenase 1; Keap1: Kelch-like ECH-associated protein 1; Nrf2: Nuclear factor-erythroid factor 2-related factor 2; PRDX6: Peroxiredoxin 6; POR: Cytochrome P450 Oxidoreductase; B2M: Beta-2 microglobulin.