The dynamic expression patterns of important genes associated with MTC (SAMSDC, SPDS) and PARylation processes (PARP, PARG and ADPR-PPase) (A–E) and PVY RNA accumulation (F) analysed by RT-qPCR in systemically infected leaves of Chicago plants at 22 or 28 °C at 8 dpi, as shown. SAMDC, adenosylmethionine decarboxylase (Soltu.DM.02G030190.1); SPDS, spermidine synthase (Soltu.DM. DM.06G014450.1); PARG, poly(ADP-ribose) glycohydrolase (Soltu.DM.12G003820.1); PARP, Poly(ADP-ribose) polymerase (Soltu.DM.03G032200.1); ADPR-PPase, ADP-ribose pyrophosphatases (Soltu.DM.08G000850.1). Analysis of variance and Tukey’s HSD post hoc tests were performed on the RT-qPCR data. ** p < 0.01, *** p < 0.001.