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. 2022 Mar 4;14(5):1087. doi: 10.3390/nu14051087

Figure 4.

Figure 4

Effects of (a) NHDC and (b) GNHDC on lipid accumulation and cell viability in 3T3-L1 cells. Lipid accumulation was measured by (A) oil red O staining (magnification 100×, scale bar 100 μm) and (B) Triacylglycerol accumulation was quantified with Ctrl, 30 μM, 50 μM, and 100 μM (a) NHDC or (b) GNHDC. (C) Cell viability in 3T3-L1 cells was evaluated with 50 μM (a) NHDC or (b) GNHDC for 8 days by MTT assay. All data are shown as the mean ± SEM and were analyzed by one-way ANOVA with a Newman–Keuls post hoc test or an unpaired Student’s t-test (n = 3). TAG, triacylglycerols; Ctrl, control; NHDC, neohesperidin dihydrochalcone; N 30, 30 μM NHDC; N 50, 50 μM NHDC; N 100, 100 μM NHDC; GNHDC, NHDC-O-glycoside; GN 30, 30 μM GNHDC; GN 50, 50 μM GNHDC; GN 100, 100 μM GNHDC. The same letter indicates no significant differences (p > 0.05) and different letters indicate significant differences (p < 0.05, ANOVA).