TABLE 1.

Oligonucleotide primers used in this study

Primer	Orientation when used as primer	Positionsa	Base sequence
VGYR-1	Sense	GyrA, 41–46; ParC, 43–48	5′-T(A/C/G/T)AA(A/G)CC(A/C/G/T)GT(A/C/G/T)CA(C/T)(A/C)G-3′
VGYR-2	Sense	GyrA, 57–62	5′-AA(C/T)GA(C/T)TGGAA(C/T)AA(A/G)(G/C)C-3′
VGYR-3	Sense	ParC, 78–83	5′-AA(A/G)TA(C/T)CA(C/T)CC(A/C/G/T)CA(C/T)G-3′
VGYR-4	Antisense	GyrA, 124–119; ParC, 127–122	5′-TC(A/C/G/T)GT(A/G)TA(A/C/G/T)C(G/T)CAT(A/C/G/T)GC-3′
GYRAS1	Antisense	564–534	5′-CGGTGTAACGCATTGCCGCAGCAGAGTCGCC-3′
GYRAS2	Antisense	395–365	5′-GGCTGATTTTTTATATGGTTTGTTCCAATCG-3′
GYRS1	Sense	365–395	5′-CGATTGGAACAAACCATATAAAAAATCAGCC-3′
GYRS2	Sense	534–564	5′-GGCGACTCTGCTGCGGCAATGCGTTACACCG-3′
PARAS1	Antisense	465–434	5′-GGAGCACCCCAGTTTCCCTGACCATCAACCAG-3′
PARAS2	Antisense	441–408	5′-TCAACCAGTGGGTAACGGTAAGAGAATGGTTGCG-3′
PARS1	Sense	403–436	5′-AATGGCGCAACCATTCTCTTACCGTTACCCACTG-3′
PARS2	Sense	434–465	5′-CTGGTTGATGGTCAGGGAAACTGGGGTGCTCC-3′
GYRCL1	Sense	11–42	5′-AAATATAGTCAGTTTCAGAAAGATATGGGATG-3′
GYRCL2	Antisense	2895–2864	5′-TGATGAAGATACAAAAAAGACGAGAATTTTGC-3′
PARCL1	Sense	11–40	5′-ATCGACGACTCTGAAGCCACTATGGAAATG-3′
PARCL2	Antisense	2523–2495	5′-GTTGGTATCATTCGTAAAGGCAAGGAATG-3′
GYRM1	Sense	365–386	5′-CGATTGGAACAAACCATATAAA-3′
GYRM2	Antisense	564–545	5′-CGGTGTAACGCATTGCCGCA-3′
PARM1	Sense	293–312	5′-CTTGGTCTTTCGGCATCAGC-3′
PARM2	Antisense	506–487	5′-CTTCGGTATAACGCATTGCC-3′
Cassette C1			5′-GTACATATTGTCGTTAGAACGCGTAATACGACTCA-3′
Cassette C2			5′-CGTTAGAACGCGTAATACGACTCACTATAGGGAGA-3′

^aThe positions of VGYR-1, VGYR-2, VGYR-3, and VGYR-4 degenerate primers correspond to amino acid residues of E. coli GyrA and ParC (Fig. 2 and Fig. 3). The positions of other primers correspond to base positions of the V. parahaemolyticus gyrA and parC genes deposited in GenBank (accession no. AB023569 and AB023570, respectively); primer designations starting with GYR and PAR indicate primers for the gyrA and parC genes, respectively.