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. 2022 Feb 28;14(5):1044. doi: 10.3390/nu14051044

Figure 2.

Figure 2

RGE suppresses reduction in phosphorylated Nrf2, HO-1, and SOD-1 levels and SOD activity in H. pylori-infected AGS cells. The cells were pretreated with the indicated concentrations of RGE for 1 h and then infected with H. pylori for 1 h. (A) The levels of Nrf2, phosphorylated Nrf2, Keap1, HO-1, SOD-1, and SOD-2 in whole-cell extracts were determined by western blotting (left panel). The ratios of Keap1/Actin, p-Nrf2/Nrf2, HO-1/Actin, SOD-1/Actin, and SOD-2/Actin were determined from band densities of these proteins. The protein band of the untreated and uninfected cells was set at 100. * p < 0.05 (right panel). The densitometry data represent the mean ± standard error (SE) from three immunoblots and are shown as the relative density of protein bands normalized to actin. (B) SOD activity in whole-cell extracts was measured using an SOD assay kit. The SOD activity in the untreated and uninfected cells was set at 100%. * p < 0.05.