Figure 7.

CC-1690 and CC-5325 had comparable resistance to the detergent Triton X-100. Detergent assay was performed to check cell wall integrity. Triton X-100 is a detergent to disrupt lipid-based membranes and cause cells without an intact cell wall to lyse. Algal samples with and without 0.05% Triton X-100 were vortexed vigorously. Strains without intact cell walls will have more broken cells in the presence of Triton X-100 and thus more released chlorophyll than strains with intact cell walls. (a) Released chlorophyll from damaged cells was quantified using absorbance at 435 nm. (b) The percentage of intact cells was quantified using a Coulter Counter. Mean ± SE, N = 3 biological replicates. Statistical analyses were performed using a two-tailed t-test assuming unequal variance by comparing with CC-1690 under the same experimental conditions (*, p < 0.05) or within the same strain in the presence or absence of Triton X-100 (#, p < 0.05; ns, not significant, p > 0.05).