Table 2.
Phenolic profile (mg kg−1 DW) of Olea europaea leaves (Olivastra Seggianese variety) under control (C) and UV-B conditions sampled at the 2nd (T2), 4th (T4), 6th (T6), and 8th (T8) week after the onset of stress. Values are mean ± standard deviation (n = 3–4). Rt—Retention time; is.—isomer. ANOVA showed that most of chemical components were significantly affected by the main factors and their interactions.
| Rt (min) | Compound | [M-H]− (m/z) |
MS2 (m/z) Fragments |
T2 | T4 | T6 | T8 | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| C | UV-B | C | UV-B | C | UV-B | C | UV-B | ||||
| Flavonoids | |||||||||||
| 9.8 | Apigenin 6,8-di-C-glucoside | 593 | 353/383/503/575 | 217.0 ± 13.0 | 225.6 ± 7.6 | 207.5 ± 18.4 | 245.3 ± 3.6 | 203.1 ± 1.4 | 271.4 ± 15.4 | 215.5 ± 6.2 | 357.0 ± 1.2 |
| 11.6 | Quercetin-3-O-rutinoside | 609 | 301 | 216.1 ± 12.5 | 222.4 ± 7.0 | 213.0 ± 12.9 | 244.6 ± 3.3 | 199.2 ± 1.1 | 269.7 ± 14.8 | 210.9 ± 6.9 | 360.2 ± 1.1 |
| 11.9 | Luteolin-7-O-rutinoside is. 1 | 593 | 285 | 224.1 ± 16.8 | 225.1 ± 8.3 | 238.4 ± 4.0 | 248.4 ± 4.4 | 217.8 ± 5.5 | 279.1 ± 16.5 | 236.3 ± 10.8 | 380.5 ± 4.3 |
| 12.1 | Luteolin-7-O-rutinoside is. 2 | 593 | 285/447 | 229.6 ± 20.6 | 226.1 ± 7.9 | 251.4 ± 8.0 | 257.7 ± 5.2 | 246.7 ± 19.4 | 292.7 ± 19.7 | 277.0 ± 22.2 | 460.9 ± 19.5 |
| 12.8 | Apigenin-7-O-rutinoside | 577 | 269 | 229.3 ± 20.4 | 252.9 ± 13.7 | 248.7 ± 10.8 | 297.1 ± 10.8 | 256.0 ± 7.7 | 356.5 ± 35.1 | 288.6 ± 19.1 | 427.3 ± 13.3 |
| 13.1 | Luteolin-4′-methyl ether | 607 | 299/284 | 225.5 ± 17.8 | 239.5 ± 11.2 | 230.4 ± 6.1 | 274.3 ± 14.1 | 225.8 ± 3.0 | 339.6 ± 46.8 | 247.9 ± 8.5 | 414.6 ± 14.4 |
| 13.4 | Luteolin-7-O-glucoside | 447 | 285 | 246.8 ± 33.5 | 250.7 ± 15.5 | 282.4 ± 16.1 | 278.0 ± 4.6 | 315.6 ± 22.0 | 321.0 ± 27.9 | 353.3 ± 30.2 | 501.1 ± 29.2 |
| 15.8 | Luteolin | 285 | 342.0 ± 112.1 | 299.7 ± 29.5 | 489.8 ± 96.0 | 449.7 ± 22.7 | 543.1 ± 104.2 | 493.9 ± 77.3 | 471.4 ± 51.2 | 702.8 ± 47.9 | |
| 17.5 | Apigenin | 269 | 225/149/201 | 228.0 ± 19.7 | 258.3 ± 15.7 | 234.8 ± 5.9 | 304.8 ± 14.9 | 228.5 ± 7.4 | 340.4 ± 31.2 | 243.5 ± 9.2 | 410.8 ± 9.2 |
| 17.8 | Diosmetin is. 1 | 299 | 284 | 270.0 ± 52.5 | 294.7 ± 24.8 | 317.2 ± 33.6 | 319.3 ± 18.7 | 359.4 ± 34.9 | 383.5 ± 37.4 | 389.1 ± 28.5 | 519.9 ± 27.1 |
| 20.1 | Diosmetin is. 2 | 299 | 284 | 221.4 ± 15.7 | 251.2 ± 14.6 | 226.9 ± 7.7 | 292.0 ± 13.8 | 224.9 ± 2.2 | 306.4 ± 24.6 | 241.7 ± 11.3 | 399.1 ± 12.5 |
| Secoiridoid | |||||||||||
| 12.5 | Caffeoyl-6′-secologanoside | 551 | 507/341/389/281 | 220.3 ± 14.5 | 232.2 ± 8.6 | 223.0 ± 8.5 | 260.8 ± 5.6 | 215.1 ± 1.7 | 315.4 ± 24.7 | 234.4 ± 10.6 | 388.3 ± 5.7 |