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. 2022 Feb 28;18(2):e1010092. doi: 10.1371/journal.pgen.1010092

Fig 3. LIN-39 regulates terminal fate markers through Hox binding motifs in their promoters.

Fig 3

(A) The effects of deleting Hox sites in gcy-32 and gcy-37 promoters on GFP reporter expression in AQR and PQR. The orange boxes represented canonical Hox sites matching the sequence 5’-TGATNNAT-3’ at the indicated position. The circles in the right images indicate the loss of expression. (B) The effects of deleting Hox sites in flp-12 and lad-2 promoters on GFP expression in SDQL and SDQR. (C) The effects of deleting Hox sites in ser-2 promoter. Purple boxes represented alternative Hox sites matching the sequence 5’-TTTG(A/T)AT(T/C)T-3’ at -266 (site #3) and -183 (site #4). (D) The effects of deleting Hox sites in F49H12.4 promoter, which included the first exon (E1) and the first intron (the sequence between E1 and GFP). Removing both sites eliminated expression in AQR, PQR, and PVD but caused ectopic GFP expression in unidentified head neurons (question mark). The quantification shows the mean ± SD for the percentage of cells expressing the GFP from at least two independent lines.