Fig 4. yhdP, tamB mutants in W3110 exhibit multiple defects associated with the cell envelope.
(A) Growth of yhdP, tamB mutants. Strains were monitored by OD600 every 30 minutes at 37°C and error bars represent SD from technical triplicates with data representative of 3 biological replicates. (B) Loss of both yhdP and tamB results in decreased cell surface area. Cell width and length of listed strains were measured using MicrobeJ software after strains were grown to mid-log phase and imaged at 1000x on agarose pads, cell surface area was calculated using cell width and length. Grey bar indicates mean. T-test used between strains. 0.0001>P****, 0.001>P***. (C) Microscopy of yhdP and tamB mutants. Phase contrast microscopy (1000x) of the indicated cells with black scale bar set to 10 μm. Image in bottom left corner of dividing cell is a 300x zoom of the larger image. Microscopy data is representative of two biological experiments. (D) The vancomycin MIC of yhdP, tamB mutants and respective control strains. MICs were determined by E-test after overnight growth at 37°C.