Fig 3. Differential expression of target genes between WT and TREM2KO iPSC cells assessed by qPCRs and RNA-Seq.
The target genes (TREM2, P2RY12, CD52, ACSS3, SYDE1, TBX10) have been normalized with the normalization factor (NF) computed from conventional reference genes RPLP0 & GUSB in the first column (A, D, G, J, M, P) or from RNA-Seq derived reference genes CNBP & KIF13A in the second column (B, E, H, K, N, Q). The third column contains the Fold Changes computed from RNA-Seq (C, F, I, L, O, R). The normalized counts for each gene in each group were used to calculate the fold changes in RNA-Seq. The WT group was used as the experimental control. The Padj value of RNA-Seq is indicated in the TREM2KO group for each gene in the RNA-Seq column. For the qPCRs, Non-parametric Mann Whitney U test was used to assess differences between the groups. The alpha value was set at 0.05 and P values are annotated as follows: * P<0.05.