Fig 4. Developmental stage-associated expression differences between fetal-like and adult heart.

(A) Estimated proportions of cell types across the 966 bulk RNA-seq samples. (B-C) Scatterplots showing the differential gene expression effect size considering cell type proportions as covariates (ridge regression, Y axis) versus without considering cell types (linear model, LM, X axis) for (B) iPSC-CVPC versus adult heart and (C) adult heart versus adult arteria. Genes differentially expressed in both analyses and have the same direction of effect are shown in green while genes with opposite direction of effect are in red; genes differentially expressed only using the LM are shown in blue; and genes that are differentially expressed only using ridge regression are in cyan.