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. 2022 Mar 9;6(1):016103. doi: 10.1063/5.0085564

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FIG. 3. — Expression of BBB marker proteins in HBMEC/ci18 cells after static and perfusion culture in the device. Confluent HBMEC/ci18 cells were cultured in the device for 72 h under static (a)–(f) or flow conditions [(a′)–(f′), 0.3 dyn/cm²]. Localization of BBB marker protein was analyzed by immunocytochemistry. Cell nuclei were counterstained with DAPI (blue). Arrows in panels (a′)–(f′) represent the flow direction. Scale bars, 50 μm.