Figure 2.

DIMT1 knockdown in INS-1832/13 and EndoC-βH1 cells and rat islets. Gene expression of DIMT1 is shown in silenced INS-1832/13 (A) and EndoC-βH1 cells (B); cells treated with scramble siRNA served as control and actin mRNA expression as an internal control. DIMT1 silencing at the protein level was determined by Western blot in INS-1832/13 (C) and EndoC-βH1 cells (D) and tubulin was used as loading control; knockdown of DIMT1 in rat islets was analyzed by q-PCR as shown in (E). Data are expressed as mean ± SD (n = 5, EndoC-βH1, n = 5, INS-1832/13 and n = 4 in rat islets) and were statistically compared with paired Student’s t test; ∗∗p < 0.01, ∗∗∗p < 0.001. DIMT1, dimethyladenosine transferase 1