Table 1.

Transformations of Saccharomyces cerevisiae MaV203 strain.

Test number	Bait plasmid	Prey plasmid	Tested pair	Purpose
0	None	None	Empty plasmids	Negative Controls
1	pDEST32/Krev1	pDEST32/RalGDS-wt	Krev1+RalGDS-wt	Strong positive interaction control
2	pDEST32/Krev1	pDEST32/RalGDS-m1	Krev1+RalGDS-m1	Weak positive interaction control
3	pDEST32/Krev1	pDEST32/RalGDS-m2	Krev1+RalGDS-m2	Negative interaction control
4	pDEST32	pDEST22	None (Empty vectors)	Negative activation control
5	pDEST32/IDS	pDEST22	IDS	Negative activation control; baseline
6	pDEST32/IDS	pDEST22/1433G	IDS+1433G	Testing specific two-hybrid interaction
7	pDEST32	pDEST22/1433G	1433G	Negative activation control
8	pDEST32/IDS	pDEST22/1433Z	IDS+1433Z	Testing specific two-hybrid interaction
9	pDEST32	pDEST22/1433Z	1433Z	Negative activation control
10	pDEST32/IDS	pDEST22/ALDOA	IDS + ALDOA	Testing specific two-hybrid interaction
11	pDEST32	pDEST22/ALDOA	ALDOA	Negative activation control
12	pDEST32/IDS	pDEST2/ALDOC	IDS + ALDOC	Testing specific two-hybrid interaction
13	pDEST32	pDEST22/ALDOC	ALDOC	Negative activation control
14	pDEST32/IDS	pDEST22/EAA1	IDS + EAA1	Testing specific two-hybrid interaction
15	pDEST32	pDEST22/EAA1	EAA1	Negative activation control
16	pDEST32/IDS	pDEST22/HSP7C	IDS + HSP7C	Testing specific two-hybrid interaction
17	pDEST32	pDEST22/HSP7C	HSP7C	Negative activation control
18	pDEST32/IDS	pDEST22/LSAMP	IDS + LSAMP	Testing specific two-hybrid interaction
19	pDEST32	pDEST22/LSAMP	LSAMP	Negative activation control
20	pDEST32/IDS	pDEST22/PRDX2	IDS + PRDX2	Testing specific two-hybrid interaction
21	pDEST32	pDEST22/PRDX2	PRDX2	Negative activation control
22	pDEST32/IDS	pDEST22/SYT1	IDS + SYT1	Testing specific two-hybrid interaction
23	pDEST32	pDEST22/SYT1	SYT1	Negative activation control