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. 2022 Jan 28;41(11):1563–1575. doi: 10.1038/s41388-022-02192-2

Fig. 3. Loss of NFE2L3 leads to the downregulation of Il33 mRNA and upregulation of transcripts linked to the RAB pathway.

Fig. 3

a Top 10 pathways resulting from Enrichr [79] enrichment analysis. b Heatmap of differentially expressed genes of the wikipathway “Deregulation of RAB and RAB Effector Genes in Bladder Cancer_Homo sapiens_WP2291” comprising the Sytl2, Rab27a, Rab27b, Sytl4, and Myrip genes [76] as well as Il33. c–h RT-qPCR expression analysis of genes shown in the heatmap (b). i RT-qPCR expression analysis of Nfe2l3 and Rab27a in NFE2L3 wild type and knockout BMMCs (n = 3 in triplicates). Eef2, Rplp0, Nono, and Tbp were used as reference genes. j Immunoblot analysis of NFE2L3 wild type and knockout BMMCs using RAB27A antibody (Abcam, ab55667) (n = 3 in triplicates). Actin was used as loading control. Data represents normalized relative expression values ± SD; Unpaired t-test between genotypes is represented in *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 and ****p ≤ 0.0001, and between normal tissue and tumor within the same genotype; ΔΔp ≤ 0.01 and ΔΔΔp ≤ 0.001.