Fig. 3.

Analysis of gene family representation and constraint for the 3381 mouse–human orthologs that currently lack a null allele in mouse. a The set of remaining genes is specifically enriched for large gene families and the RIKEN cDNA collections. Olfactory receptors and zinc finger proteins are the most highly represented gene families. b Classification of the remaining genes using human orthologs to assess cell essentiality based upon CRISPR/Cas9 screens in cancer cell lines and functional constraint using probability of loss-of-intolerance (pLI) scores that range from 0 to 1 with values closer to 1 associated with higher level of constraint