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. 2022 Mar 10;13:1248. doi: 10.1038/s41467-022-28913-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a C57BL/6 mice pretreated with 3-day antibiotics were administrated with F. nucleatum or E. coli everyday by gavage. Mice were sacrificed after the treatment for 15 days. b Quantitative RT-PCR analysis of F. nucleatum in colorectum tissues from the indicated mice (n = 6). c, d Quantitative RT-PCR analysis of METTL3 mRNA (c) and KIF26B mRNA (d) expression in colorectum tissues from the indicated mice (n = 6). e Quantitative RT-PCR analysis of F. nucleatum in human CRC tissues and matched adjacent non-tumor tissues. The CRC patients in this cohort all had lymph node metastasis (n = 26). f, g Quantitative RT-PCR analysis of METTL3 (f) and KIF26B (g) in human CRC tissues and matched adjacent non-tumor tissues. h, i The correlation between the relative levels of F. nucleatum 16 S and METTL3 mRNA (h) or the correlation between the levels of METTL3 and KIF26B mRNA (i) in CRC tissues and adjacent non-tumor tissues (n = 26). The correlation was analyzed by linear regression. j Kaplan–Meier survival curves were analyzed and compared between patients with low (n = 129) and high (n = 129) levels of KIF26B in CRC patients from The Cancer Genome Atlas (TCGA) database. Subgroups with high- or low-KIF26B expression were sorted according to the TPM gene expression standard values. k Multivariable analysis was performed of CRC patients in the TCGA database. The center dots represent the Hazard ratio (HR). All the bars correspond to 95% confidence intervals. P value, multivariate cox regression analysis. The details were provided in the methods section. l Schematic diagram of the F. nucleatum-induced downregulation of METTL3-mediated m⁶A modifications contributes to CRC metastasis. Data of quantitative RT-PCR are presented as log₂ value normalized to universal Eubacteria 16 S (b), GAPDH (c, d, f, g), or PGT (e). Data were shown as mean ± SD. P values were shown. Two-tailed Mann–Whitney test (b–d), Two-tailed paired t-test (e–g), and log-rank test (j). AJCC American Joint Committee on Cancer, CI confidence interval, HR hazard rate.