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. 2022 Mar 10;13(3):223. doi: 10.1038/s41419-022-04645-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A The proportions of CXCR5 + TIM3− cells among TPX2^low and TPX2^high (indicated in Fig. 1D) tumor-infiltrating CD8+ T cells were analyzed by flow cytometry. B IF staining of CXCR5 and CD8 in the corresponding TPX2^low and TPX2^high HCC samples (indicated in Fig. 1A). Scale bar: 50 μm. C IF staining of TPX2 and CXCR5 in tumor-infiltrating CD8+ T cells transfected with LV-TPX2 or LV-NC. Scale bar: 5 μm. D, E The expression of CXCR5 was detected in tumor-infiltrating CD8+ T cells transfected with LV-TPX2 or LV-NC and PBMC-derived CD8 + T cells transfected with LV-shRNA or LV-NC. ***p < 0.001; the two-tailed unpaired Student’s t-test was used to compare two groups. CXCR5 C-X-C chemokine receptor type 5, DAPI 4′,6-diamidino-2-phenylindole, for nuclear staining, LV-NC lentivirus used as a control, LV-shRNA lentivirus used to knock down the human TPX2 gene, LV-TPX2 lentivirus used to overexpress the human TPX2 gene, PBMCs peripheral blood mononuclear cells, p-P65 phosphorylated P65, TIL tumor-infiltrating lymphocyte, TIM-3 T cell immunoglobulin and mucin domain containing-3, TPX2 microtubule nucleation factor. Raw western blot results can be found in the Original data file.