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. 2022 Mar 10;13(3):223. doi: 10.1038/s41419-022-04645-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A, B The expression of TPX2, CXCR5, NF-κB, and p-P65 was detected in tumor-infiltrating CD8 + T cells transfected with LV-TPX2 or LV-NC and/or treated with an inhibitor of P65 phosphorylation (BAY 11-7802). C The expression of TPX2, CXCR5, NF-κB, and p-P65 was detected in tumor-infiltrating CD8+ T cells treated with PMA or TNF. D Co-IP (coimmunoprecipitation) analysis of TPX2 and p-P65. ***p < 0.001; the two-tailed unpaired Student’s t-test was used to compare two groups. CXCR5 C-X-C chemokine receptor type 5, co-IP coimmunoprecipitation, LV-NC lentivirus used as a control, LV-TPX2 lentivirus used to overexpress the human TPX2 gene, NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells, PBMCs peripheral blood mononuclear cells, p-P65 phosphorylated P65, TIL tumor-infiltrating lymphocyte, TPX2 microtubule nucleation factor. Raw western blot results can be found in the Original data file.