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. 2022 Mar 10;13:1241. doi: 10.1038/s41467-022-28757-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a GII.4 HOV VLP cryo-EM structure aligned to the crystal structure shown in Fig. 1 and viewed along the icosahedral twofold axis, shows the T = 3 symmetry of GII.4 HOV VLP in solution. The subunits A, B, and C are colored yellow, blue, and pink. b The surface and cut-away views of a GII.4 cryo-EM map colored by radial distance, showing similar particle radius as observed in the crystal structure. c Superimposition of subunit A (orange) with B (blue) or C (magenta) subunits of the cryo-EM structure with that of the VLP crystal structure (subunit A, yellow; subunit B, light blue; subunit C, pink) using the S domain. The N-terminus of VP1 in the cryo-EM and crystal structure is labeled with colored and black “N“s.